Changes in peroxidase and polypeptide profiles in Nicotiana tabacum L. transformed with Agrobacterium rhizogenes
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منابع مشابه
Alkaloids in Stem Roots of Nicotiana tabacum and Spartium junceum Transformed by Agrobacterium rhizogenes
Gesellschaft für Biotechnologische Forschung, Mascheroder Weg, D-3300 Braunschweig-Stöckheim, Bundesrepublik Deutschland Z. Naturforsch. 42c, 69—72 (1987); received August 21/September 22, 1986 Agrobacterium rhizogenes, Alkaloids, Nicotine, Cytisine, Nicotiana, Spartium The growth of root hairs from stems of Nicotiana tabacum and Spartium junceum was induced by Agrobacterium rhizogenes. Stem ro...
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Here, efficient plantlet regeneration via somatic embryogenesis from Panax quinquefolium hairy roots was reported. Callus development was optimal on MS medium containing 2,4-dichlorophenoxyacetic acid (1 mg l) and -naphthalene acetic acid (1 mg l). After a 4-week growth, calli were transferred onto MS medium with different combinations of plant growth regulators (PGRs). The highest embryogenesi...
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1. Coproporphyrinogenase was extracted and purified from tobacco (Nicotiana tabacum L.). Enzyme activity was mainly located in mitochondria rather than in chloroplasts. The enzyme was purified by differential centrifugation, ammonium sulphate fractionation, calcium phosphate gel adsorption and dialysis. A 69-fold final purification was obtained. 2. An apparent K(m) value of 3.6x10(-5)m was foun...
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To improve Agrobacterium-mediated transformation of tobacco, factors influencing gene delivery, including genotype of the plant, bacterial strain, and Agrobacterium transformation procedure, were tested via direct somatic embryogenesis. Leaf tissue of three different tobacco genotypes (Nicotiana tabacum L. cvs. Samsun, and Xanthi, and N. benthamiana) we...
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Two kinds of cellular responses to auxin, the hyperpolarization of protoplasts and the division of protoplast-derived cells, were compared in Nicotiana tabacum plants transformed by different T-DNA fragments of Agrobacterium rhizogenes strain A4. Using transmembrane potential difference measurements to characterize hormonal sensitivity of mesophyll protoplasts, we found a 30-fold increase in se...
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ژورنال
عنوان ژورنال: Ciência Rural
سال: 1995
ISSN: 0103-8478
DOI: 10.1590/s0103-84781995000200009